MicroTAS 2017

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Workshop 2 (Morning 9:00 - 12:00)

Title: LAB-ON-A-CHIP (LOC) TECHNOLOGIES FOR THE ISOLATION OF CIRCULATING MARKERS: APPLICATION IN LIQUID BIOPSIES

Presenter: Steven Soper, University of Kansas, USA

Workshop Description: Purpose: The goal of this short course is to introduce participants into the burgeoning area of liquid biopsies and the technologies used for the isolation of the markers (Circulating Tumor Cells, CTCs, cell-free DNA, cfDNA, and exosomes) directly from whole blood comprising the liquid biopsy. The major technology platform that will be discussed includes lab-on-a-chip (LOC) platforms. This short course will be broken down into two general areas: (1) Operational characteristics of microfluidics for selecting rare markers from whole blood; and (2) applications of microfluidic technologies for the selection of rare circulating biomarkers from whole blood.

Outline:
  1. Basic fundamentals of LOC platforms
    1. Brief introduction to the analytical challenges with the isolation of circulating markers from whole blood: CTCs, cfDNA and exosomes
    2. Introduction to LOC technologies; what can they provide with respect to the analysis of rare circulating markers and comparison to benchtop procedures.
    3. Materials for LOC
    4. Fabrication technologies
    5. Metrology and fluid characteristics in microfluidics
  2. Application of LOC technologies for the isolation of circulating markers from whole blood.
    1. Basic components of the liquid biopsy (CTCs, cfDNA and Exosomes)


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