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Exhibition Current Support Booth Availability Exhibition Schedule Live Labs (NEW!) Industrial Stage (NEW!)  Exhibitor Prospectus<< Back |
Live Lab 2 THE PASCA SINGLE CELL MANIPULATOR: AUTOMATED PRINTING OF INDIVIDUAL LIVING CELLS FOR A VARIETY OF DOWNSTREAM ANALYSIS TECHNIQUES Presenters: Jonas Schoendube, MSc, R&D Engineer Andre Gross, Dipl. Ing., R&D Engineer Time: Tuesday 29 October, 09:30 - 11:30 PASCA Georges-Koehler-Allee 103 Freiburg, 79110 GERMANY phone: +49-761-203-73260 fax: +49-761-203-73299 email: jonas.schoendube@imtek.de www.pasca.eu Separation and manipulation of cells for single cell analysis remains challenging for many life science applications, including single cell genomics, isolation of circulating tumor cells (CTC) and clonal cell line production. In order to provide a platform technology to deliver single living cells to such applications, we have developed a liquid handling instrument termed single cell manipulator (SCM). The SCM is able to print single living cells confined in free flying droplets in an ink-jet like manner into well plates, microfluidic chips or other substrates for subsequent single cell applications. A machine vision system detects single cells in proximity of the nozzle before droplet ejection. Droplets, predicted to contain no cell or more than one cell, are deflected by a pneumatic shutter system. Only droplets with exactly one single cell are printed onto the substrate. The SCM will be presented for the first time in a hands-on demonstration at MicroTAS 2013. SCM's are already available for selected pilot partners (industry and research) and have been applied to isolate and sort various cell types, including primary human stem cells, primary mouse neurons, human blood and cancer cells. Furthermore it perfectly fits to a variety of downstream applications within the scope of the MicroTAS conference, which makes the SCM technology a great addition to the sessions 3A3, 3A4, 1B3, 4B2. Participants of the PASCA LiveLab will get hands-on experience on a fully working SCM. To eliminate issues of biological safety, polymer particles will be used to model cells. After a short introduction to the technology, two standardized experiments (single cell plating in wells and single cell array printing) will be performed. A bench-top microscope will be provided to allow for evaluation of the results. Finally there will be time to discuss customer/participant needs. |
