LIVE CELL IMAGING IN MICROFLUIDICS
Tom Lummen, ETH Zürich, Basel, SWITZERLAND
Oliver Biehlmaier, University Basel, SWITZERLAND
Gregor Schmidt, ETH Zürich, Basel, SWITZERLAND

Workshop Description:
Lectures with hands-on session and discussion rounds.

Overview of Material to Be Covered and What Attendees Can Expect to Take Away From the Workshop:

Getting and keeping your cells in focus - basic microscopy

• Physics of microscopy
• Optimizing imaging for microfluidics
• Live cell imaging setups

Fluorescent labelling and imaging of live cells

• Fluorescence basics and imaging technologies
• Selecting dyes and fluorescent proteins for your assay

Tackling phototoxicity in live cell imaging

• What can go wrong?
• How to detect of phototoxicity
• Optimize the experiment to minimize phototoxicity

Who Should Attend:
The workshop is targeted at students (Master or PhD) planning or performing fluorescence microscopy of live cells. Prior knowledge is not required.